PROMOTIONAL FEATURE
EXOSOMES AND Bio-Pulsing Technology
A Scientific Framework for Advancing Regenerative Aesthetics
ABSTRACT
Exosomes, a subset of extracellular vesicles (EVs), are key mediators of intercellular communication and tissue repair. Their ability to transport proteins, mRNA, microRNA (miRNA), and lipids positions them as promising tools in regenerative and aesthetic medicine. Yet, conventional production faces hurdles in scalability, purity, and stability. This paper introduces Bio-Pulsing technology, a novel method for optimising yield and therapeutic efficacy of exosomes derived from non-human mesenchymal stem cells (MSCs). Preclinical and early clinical findings are reviewed with focus on dermatological and trichological applications. Bio-Pulsing is a patented AM Biotech process for LA EXO exosomes.
1. INTRODUCTION
Exosomes are nanoscale vesicles (30–150 nm) secreted by most cell types, including MSCs. Their paracrine signalling regulates inflammation, immune responses, and tissue regeneration. With low immunogenicity and capacity to cross barriers, exosomes are advancing as acellular therapeutics.
In aesthetics, exosomes have been studied for their ability to:
• Stimulate fibroblast proliferation
• Remodel ECM through collagen and elastin synthesis
• Modulate melanogenesis and inflammation
• Reactivate dormant hair follicles
Despite potential, exosome therapies face bottlenecks in consistency, scalability, and stability.
2. LIMITATIONS OF CONVENTIONAL EXOSOME PRODUCTION
Isolation techniques such as ultracentrifugation, filtration, or precipitation yield heterogeneous products that may include residual proteins or apoptotic bodies. Key limitations include:
• Low yield: MSCs secrete exosomes at modest baseline levels
• Variable purity: Non-exosomal contaminants affect reproducibility
• Instability: Cold-chain dependence inflates costs and limits logistics
These issues necessitate new bioprocessing methods that maintain bioactivity while improving scalability and shelf life.
3. BIO-PULSING: MECHANISM AND MOLECULAR RATIONALE
Bio-Pulsing (AM Biotech / LA EXO) is a proprietary preconditioning technique that stimulates pathogen-free MSCs from embryonic chicken tissue with botanical compounds, such as Polygonum multiflorum. This induces a regenerative phenotype. 3.1 Yield Enhancement Stimulation upregulates RAB27A, a GTPase central to exosome release, producing up to 93-fold higher output. 3.2 Cargo Enrichment Bio-Pulsed exosomes are enriched with regenerative miRNAs and ECM-related proteins, including:
• Fibroblast proliferation: miR-21, miR-146a
• Anti-inflammatory signaling: miR-155 downregulation
• Hair follicle cycling & angiogenesis 3.3 Purification and Stability Exosomes undergo multi-step filtration and lyophilisation, achieving >99.99% purity and up to 3 years of room-temperature stability, eliminating the need for cryopreservation.
4. PRECLINICAL EFFICACY DATA
In vitro studies of Bio-Pulsed exosomes demonstrated:
Results support enhanced ECM remodelling and follicle reactivation.
5. CLINICAL EVALUATION
A 2025 IRB-approved study assessed topical LA EXO Bio-Pulsed exosomes in 60 adults (30–65 years). The formulation was a lyophilised ampoule plus essence of standardised avian MSC-derived small EVs.
• Dermatology: Wrinkle depth 7.3%; collagen density 18.2%
• Trichology: Anagen/telogen ratio 48.7%; shedding 46.5%
• Safety: No adverse events; 100% study completion
• Molecular correlates: Upregulation of regenerative markers with reduced inflammatory cytokines
6. DISCUSSION
The Bio-Pulsing platform addresses key shortcomings of conventional exosome production through high yield, stability, and molecular optimisation. This cell-free, scalable approach preserves regenerative benefits of stem cell therapies while bypassing ethical and immunologic challenges.
7. CONCLUSION
LA EXO Bio-Pulsed exosomes show strong preclinical and clinical promise in skin rejuvenation, scar modulation, and hair restoration. With enhanced stability and scalability, they represent a next-generation alternative to cell-based therapies in regenerative aesthetics.